Journal: bioRxiv
Article Title: High-content live-cell time-lapse imaging predicts cells about to die via apoptosis
doi: 10.1101/2025.10.23.684203
Figure Lengend Snippet: (A) The platemap showing replicates of ten different doses of staurosporine, an apoptosis-inducing compound. We imaged each well at four fields of view (FOVs) at three z slices, each 3um apart. Images were acquired every 30 minutes for 360 minutes (six hours), capturing Hoechst, ChromaLIVE 488_yellow, ChromaLIVE 488_red, and ChromaLIVE 561. We fixed cells at six hours and stained for AnnexinV, an apoptotic marker, and Hoechst. (B) Our HCLTI pipeline for processing time-lapse microscopy images. (C) Representative images of tracked HeLa single cells across time for varying doses of staurosporine. Cyan represents nuclei, yellow represents ChromaLive 561, and Magenta represents ChromaLive 488 at both its emission channels for visualization purposes only. Scale bars = 10 um.
Article Snippet: – We then applied the Live Cell Painting assay (ChromaLIVE TM ) , and performed live-cell time-lapse spinning disk confocal microscopy (Yokogawa CQ1), acquiring images per field of view (FOV) every 30 minutes in three z stacks for six hours.
Techniques: Staining, Marker, Time-lapse Microscopy